This Guidance Document describes the main features of a semi-field test method for the quantitative assessment of adverse effects of pesticides and other chemicals on honey bee brood development under more realistic exposure conditions and application procedures that are used for laboratory-based studies. This version is the second edition of the Guidance Document, initially published by the OECD in 2012, and has been enhanced and improved based on experience gained from using the test method. The project was on the Test Guidelines Programme work plan until 2024.