Non-OECD in vitro screens (Conceptual Framework Level 2)

Specific guidance is provided for in vitro screening assays that have been validated at a national level by the US Environmental Protection Agency (Androgen Receptor Binding Assay and the Aromatase Assay). Though these assays are not validated OECD test guidelines, they provide information on the potential mechanism of test chemicals and may be useful for evaluating chemicals as endocrine disrupters in a weight of evidence approach. For each assay, background, considerations, limitations, and when the assay might be used are discussed. Scenarios for interpreting positive, negative, and equivocal results, along with other endocrine test guideline data are also provided